Food Ingredients From Stevia Rebaudiana

ABSTRACT

Various ingredients and compositions are prepared from  Stevia rebaudiana  Bertoni plant. The compositions can be used as hulking agents, and sweeteners in foods, beverages, cosmetics and pharmaceuticals,

RELATED APPLICATION

This application is a divisional application of U.S. patent applicationSer. No. 13/530,113 entitled “Food Ingredients From Stevia Rebaudiana”,filed Jun. 22, 2012, which in turn claims the benefit under 35 U.S.C.119(e) of U.S. provisional application Ser. No. 61/500,598 entitled“Food Ingredients From Stevia Rebaudiana”, filed Jun. 23, 2011, whichare herein incorporated by references.

FIELD OF THE INVENTION

The invention relates to a process for producing food ingredients fromStevia rebaudiana Bertoni plant and their use in various food productsand beverages.

BACKGROUND OF THE INVENTION

Nowadays sugar alternatives are receiving increasing attention due toawareness of many diseases in conjunction with consumption of high-sugarfoods and beverages. However many artificial sweeteners such as dulcin,sodium cyclamate and saccharin were banned or restricted in somecountries due to concerns on their safety. Therefore non-caloricsweeteners of natural origin are becoming increasingly popular. Thesweet herb Stevia rebaudiana Bertoni produces a number of diterpeneglycosides which feature high intensity sweetness and sensory propertiessuperior to that of many other high potency sweeteners.

The above-mentioned sweet glycosides have a common aglycon, steviol, anddiffer by the number and type of carbohydrate residues at the C13 andC19 positions. The leaves of Stevia are able to accumulate up to 10-20%(on dry weight basis) steviol glycosides. The major glycosides found inStevia leaves are rebaudioside A (2-10%), stevioside (2-10%), andrebaudioside C (1-2%). Other glycosides such as rebaudioside 13, D, E,and F, steviolbioside and rubusoside are found at much lower levels(approx. 0-0.2%).

Methods for the extraction and purification of sweet glycosides from theStevia rebaudiana plant using water or organic solvents are describedin, for example, U.S. Pat. Nos. 4,361,697; 4,082,858; 4,892,938;5,972,120; 5,962,678; 7,838,044 and 7,862,845.

As it is well known the use of high intensity sweeteners in variousapplications requires various bulking agents to substitute the sugarwhich is removed from the formulation. The bulking agents used in thoseapplications include both caloric and non-calorie materials. Nonlimiting examples of bulking agents include fructooligosaccharides,inulin, inulooligosaccharides, maltooligosaccharides, maltodextins,cyclodextrins, corn syrup solids, erythritol and other sugar alcohols,glucose, maltose, lactose, tagatose, lactulose, palatinose, isomalt,modified starches etc.

Obviously more preferable are the bulking agents which provide zerocalories, such as erythritol, isomalt, fructooloigosacharides, inulinetc.

On the other hand it has to be noted that steviol glycosides arecompounds extracted from the plant and in process of their manufacturelarge amounts of “empty” biomass is created. Moreover, generally, theextraction process utilizes only the Stevia plant leaves. Thisadditionally generates large amount of the stems which have limited useas well. The “empty” biomass is mainly discharged directly toenvironment. In some cases it is used for biogas production. It might beused as biofertilizer as well. The stems are generally used as fuel.

There is no reports to-date on processing the stevia biomass into anyfood ingredient. Nevertheless, if accomplished in large scale, this canprovide significant economic, and environmental benefits as it canprovide an opportunity for inclusion of whole stevia plant into foodchain, creating practically wasteless stevia processing.

Within the description of this invention we will show that, stevia plantbiomass may be used as a source for producing valuable food ingredient,which can be used in number of food and beverage applications.

SUMMARY OF THE INVENTION

The present invention is aimed to overcome the disadvantages of existingStevia processing industrial schemes. The invention describes a processfor producing a high value food ingredient from the Stevia rebaudianaBertoni plant and use thereof in various food products and beverages asa carrier or bulking agent.

The invention, in part, pertains to a food ingredient comprisingcellulose, or cellulose containing biomass derived from Steviarebaudiana Bertoni plant.

In the invention, Stevia rebaudiana Bertoni plants, particularly theleaves and stems, were used as a starting material.

The starting material was subjected to size reduction, by means ofrotary blade milling machine. The grinded biomass was subjected toalkaline pulping, followed by chlorine-free bleaching and acidhydrolysis and spray drying to produce a microcrystalline cellulosecomplying to specifications prepared at the 55th JECFA (2000) andpublished in FNPS2 Add 8 (2000).

The obtained products are applicable to various foods and beverages asbulking agent, including tabletop sweeteners, soft drinks, ice cream,cookies, bread, fruit juices, milk products, baked goods andconfectionary products.

Preferably the product of invention is used with other sweeteners,flavors and food ingredients.

Non-limiting examples of sweeteners include steviol glycosides,stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudiosideD, rebaudioside F, rebaudioside F, dulcoside A, steviolbioside,rubusoside, as well as other steviol glycosides found in Steviarebaudiana Bertoni plant and mixtures thereof, stevia extract, Luo HanGuo extract, mogrosides, high-fructose corn syrup, corn syrup, invertsugar, fructooligosaccharides, inulin, inulooligosaccharides, couplingsugar, maltooligosaccharides, maltodextins, corn syrup solids, glucose,maltose, sucrose, lactose, aspartame, saccharin, sueralose, sugaralcohols.

It is to be understood that both the foregoing general description andthe following detailed description are exemplary and explanatory and areintended to provide further explanation of the invention as claimed.

DETAILED DESCRIPTION OF THE INVENTION

Advantages of the present invention will become more apparent from thedetailed description given hereinafter. However, it should be understoodthat the detailed description and specific examples, while indicatingpreferred embodiments of the invention, are given by way of illustrationonly, since various changes and modifications within the spirit andscope of the invention will become apparent to those skilled in the artfrom this detailed description.

In the invention, Stevia rebaudiana Bertoni plants, particularly theleaves and stems, were used as a starting material. The “empty” leafbiomass obtained after extraction of steviol glycosides, as well asStevia rebaudiana Bertoni plant stems remaining after “stripping-off”the leaves can be used as a starting material. Therefore, the steviabiomass suitable for the process of the present invention includespre-processed Stevia rebaudiana Bertoni plants (e.g., post-extraction ofsteviol glycosides) and un-processed Stevia rebaudiana Bertoni plants.

The testing of obtained microcrystalline cellulose was conductedaccording to procedures set in microcrystalline cellulose monographprepared at the 55th FAO/JECFA meeting (2000) and published in Food andNutrition Paper 52 Addendum 8 (2000).

In one embodiment of the invention, the starting material is milledusing rotary blade grinder to produce particles from 1-50 mm, preferably5-20 mm in length. Any other equipment capable of reducing largeparticles into smaller ones can be used in this stage as well.

In one embodiment of the invention the obtained grinded biomass isdispersed in the water and boiled at 100° C. for 0.5-3 hours, preferably1-1.5 hours, followed by separation of the liquid and biomass. Theprocess is repeated several times until colorless or substantiallycolorless liquid is obtained. A continuous extracting apparatus, as wellas any other equipment known to those skilled in art, may be used forthis purpose.

In one embodiment of this invention the separated biomass issubsequently introduced into 10-20% NaOH aqueous solution. The solutionis treated in pressurized vessel at 110-180° C., preferably 120-170° C.,during 0.1-10 hours, preferably 1-5 hrs to produce pulped biomass.Alternatively other pulping techniques such as biological or organicsolvent pulping, mechanical, thermomechanical, chemo-thermomechanical,chemical pulping such as Kraft process, sulfite process can be used.After thermal treatment the pulped biomass is separated from the liquidand washed with the water till neutral pH of washing water is achieved.

In one embodiment of this invention the washed biomass is treated withbleaching agent. Various bleaching agents such as hydrogen peroxide,ozone, chlorine, sodium hypochlorite, chlorine oxide, enzymes may beused. However it is preferred to use chlorine-free bleaching agents.

In one embodiment of present invention the bleaching of pulped biomassis achieved by using 10-30%, preferably 20-25%, hydrogen peroxide. Thebiomass was suspended in 0.5-5, preferably 1-3 volumes of hydrogenperoxide and incubated at 80-150° C., preferably 100-120° C., during0.1-3 hours, preferably 0.5-1.5 hours. The bleached biomass wassubsequently separated from liquid and washed with water.

In one embodiment of this invention, the obtained biomass was subjectedto partial depolimerization. Various agents capable of depolimerizingthe cellulose, such as mineral acids and enzymes, can be utilized forthis stage.

In one embodiment of this invention the depolimerization is achieved by1-20%, preferably 5-15% hydrochloric acid. The ratio of bleached biomassto acid solution is 1:0.5 to 1:5, preferably 1:1 to 1:3. The temperatureof depolimerization process is 50-120° C., preferably 80-100° C. during0.1-5 hours, preferably 0.5-2 hours.

In one embodiment the obtained depolimerized cellulose is separated fromacid solution and washed with water until neutral pH is achieved. Theobtained solids are suspended in 1-3 volumes of water and spray dried atinlet temperature of 180° C. and outlet temperature of 100° C.

In one embodiment of present invention a stevia sweetener is added todepolimerized cellulose slurry. The ratio of stevia sweetener todepolimerized cellulose on dry weigh basis is 1:50 to 1:400 (wt),preferably 1:100 to 1:300.

Any other drying techniques such as flash drying, or vacuum drying canbe used for the drying of the slurry as well.

Non-limiting examples of stevia sweeteners include stevia extract,steviol glycosides, stevioside, rebaudioside A, rebaudioside B,rebaudioside C, rebaudioside rebaudioside F, rebaudioside F, dulcosideA, steviolbioside, rubusoside, as well as other steviol glycosides foundin Stevia rebaudiana Bertoni plant and mixtures thereof.

Alternatively the cellulose produced by the method of this invention canbe processed into other food ingredients by techniques known to thoseskilled in art. Non limiting examples of such ingredients include, watersoluble cellulose, carboxymethyl cellulose, methylcellulose,hydroxypropylcellulose, hydroxypropyl-methylcellulose,ethyl-methylcellulose as well as cellobiose, glucose etc.

The products of present invention can be used as bulking agents,sweeteners, flavor enhancers in various food and beverage products.Non-limiting examples of food and beverage products include tabletopsweeteners, carbonated soft drinks, ready to drink beverages, energydrinks, isotonic drinks, low-calorie drinks, zero-calorie drinks, sportsdrinks, teas, fruit and vegetable juices, juice drinks, dairy drinks,yoghurt drinks, alcohol beverages, powdered beverages, bakery products,cookies, biscuits, baking mixes, cereals, confectioneries, candies,toffees, chewing gum, dairy products, flavored milk, yoghurts, flavoredyoghurts, cultured milk, soy sauce and other soy base products, saladdressings, mayonnaise, vinegar, frozen-desserts, meat products,fish-meat products, bottled and canned foods, fruits and vegetables.

Additionally the products can be used in drug or pharmaceuticalpreparations and cosmetics, including but not limited to toothpaste,mouthwash, cough syrup, chewable tablets, lozenges, vitaminpreparations, and the like.

The products can be used “as-is” or in combination with othersweeteners, flavors and food ingredients.

Non-limiting examples of sweeteners include steviol glycosides,stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudiosideD, rebaudioside E, rebaudioside F, dulcoside A, steviolbioside,rubusoside, as well as other steviol glycosides found in Steviarebaudiana Bertoni plant and mixtures thereof, stevia extract, Luo HanGuo extract, mogrosides, high-fructose corn syrup, corn syrup, invertsugar, fructooligosaccharides, inulin, inulooligosaccharides, couplingsugar, maltooligosaccharides, maltodextins, corn syrup solids, glucose,maltose, sucrose, lactose, aspartame, saccharin, sucralose, sugaralcohols.

Non-limiting examples of flavors include lemon, orange, fruit, banana,grape, pear, pineapple, bitter almond, cola, cinnamon, sugar, cottoncandy, vanilla flavors.

Non-limiting examples of other food ingredients include flavors,acidulants, organic and amino acids, coloring agents, hulking agents,modified starches, gums, texturizers, preservatives, antioxidants,emulsifiers, stabilisers, thickeners, gelling agents.

The following examples illustrate various embodiments of the invention.It will be understood that the invention is not limited to thematerials, proportions, conditions and procedures set forth in theexamples, which are only illustrative.

EXAMPLE 1 Preparation of Stevia Ingredient

1 kg of Stevia rebaudiana plant dried leaves were grinded into pieces ofapprox 10 mm, suspended in 5 L of water and boiled for 1 hour. Theliquid was removed and the separated biomass was resuspended in thewater and treated as described above, The process was repeated 5 times.The resulted biomass was suspended in 3 L 15% NaOH solution and placedinto autoclave for pulping at 150° C. for 2 hrs. The obtained pulpedbiomass is separated from liquid and washed with deionized water tillneutral pH of washing water. The washed pulp was suspended in 3 L of 20%hydrogen peroxide and treated for 1 hr at 100° C. The bleached pulp waswashed with water and suspended in 3 L 10% hydrochloric acid. Themixture was heated to 90° C. for 1 hr. The obtained mixture was strainedthrough 60 mesh sieve and then filtered through grade 1 filter paper.The solids recovered on the filter paper were washed with deionozedwater till neutral pH of washing water was achieved. The washed weresuspended in 1.5 L of water and spray dried by YC-015 laboratory spraydrier (Shanghai Pilotech Instrument & Equipment Co. Ltd., China)operating at 180° C. inlet and 100° C. outlet temperature. About 480 gof free flowing microcrystalline cellulose was obtained.

EXAMPLE 2 Preparation of Stevia Ingredient

1 kg of Stevia rebaudiana plant dried stems were processed similarly toleaves according to EXAMPLE 1. About 670 g of free flowingmicrocrystalline cellulose was obtained.

EXAMPLE 3 Preparation of Stevia Composition

About 500 g of microcrystalline cellulose prepared according to example1 or 2 was added to 1500 mL water solution containing 2.5 g ofrebaudioside A produced by PureCircle Sdn. Bhd. (Malaysia) with purityof 99.5% (dry basis). The mixture was spray dried to yield about 490 gof dry powder.

It is to be understood that the foregoing descriptions and specificembodiments shown herein are merely illustrative of the best mode of theinvention and the principles thereof, and that modifications andadditions may be easily made by those skilled in the art withoutdeparting for the spirit and scope of the invention, which is thereforeunderstood to be limited only by the scope of the appended claims.

We claim:
 1. A sweetener composition, comprising: cellulose; and a sweetener; wherein the sweetener and cellulose has a ratio of 1:50 to 1:400 (w/w).
 2. The sweetener composition of claim 1, wherein the cellulose is obtained from a stevia biomass by a process, said process comprising the steps of: (a) providing the stevia biomass; (b) soaking the stevia biomass in water to remove water soluble components and then separating them into water-insoluble stevia biomass and first water-based filtrate; (c) incubating the water-insoluble stevia biomass from step (b) in an alkaline solution to produce a pulp; (d) washing the pulp from step (c) with water and then separating them into water-insoluble washed pulp and second water-based filtrate; (e) bleaching the washed pulp from step (d) with a chlorine-free bleaching agent to produce a bleached pulp; and separating the bleached pulp from the bleaching agent; (f) washing the bleached pulp from step (e) with water and then separating them into water-insoluble bleached pulp containing cellulose and third water-based filtrate; (g) depolymerizing the cellulose of the bleached pulp from step (f) to obtain a depolymerized pulp; (h) washing the depolymerized pulp from step (g) to yield cellulose crystals; and (i) suspending the cellulose crystals from step (h) in water and spray-drying to yield the cellulose.
 3. The sweetener composition of claim 1, wherein the sweetener is selected from the group consisting of stevia extract, steviol glycosides, stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside dulcoside A, steviolbioside, rubusoside, other steviol glycosides found in Stevia rebaudiana Bertoni plant and mixtures thereof, Luo Han Guo extract, mogrosides, high-fructose corn syrup, corn syrup, invert sugar, fructooligosaccharides, inulin, inulooligosaccharides, coupling sugar, maltooligosaccharides, maltodextins, corn syrup solids, glucose, maltose, sucrose, lactose, aspartame, saccharin, sucralose, sugar alcohols, and a combination thereof.
 4. The sweetener composition of claim 1, wherein the sweetener composition is prepared by the following steps: providing a solution of the sweetener; suspending the cellulose in the sweetener solution to obtain a mixture; spray-drying the mixture to obtain the sweetener composition.
 5. A product, comprising the sweetener composition of claim
 1. 6. The product of claim 5, further comprising a flavoring agent selected from the group consisting of lemon, orange, fruit, banana, grape, pear, pineapple, mango, bitter almond, cola, cinnamon, sugar, cotton candy, vanilla, and a combination thereof.
 7. The product of claim 5, further comprising a food ingredient selected from the group consisting of acidulants, organic and amino acids, coloring agents, bulking agents, modified starches, gums, texturizers, preservatives, antioxidants, emulsifiers, stabilisers, thickeners, gelling agents, and a combination thereof.
 8. The product of claim 5, wherein the product is food, beverage, cosmetics or pharmaceutics. 